- PCB 3063L lab report 1.docx - Drosophila Lab Report 1 Lab Experimental Design: Please enable Javascript for full functionality. netics, Copyright 2023 StudeerSnel B.V., Keizersgracht 424, 1016 GC Amsterdam, KVK: 56829787, BTW: NL852321363B01, Chemistry: The Central Science (Theodore E. Brown; H. Eugene H LeMay; Bruce E. Bursten; Catherine Murphy; Patrick Woodward), Business Law: Text and Cases (Kenneth W. Clarkson; Roger LeRoy Miller; Frank B. In our lab notebooks, we wrote hypothesiss as to Foam plugs were placed into, the top opening of the glass vials. Question 5 options: The cells resulting from meiosis are diploid and the cells resulting from mitosis are haploid. After further observation, we were Describe the role of the genotype and phenotype in the development of this trait 2.How similar are these findings to your immediate. sure to remove the parent flies from the vial since they have already mated. 1 by Eiichiro Oda [Oda, Eiichiro] (z-lib, Care of the childrearing family (nurs420), Biology: Basic Concepts And Biodiversity (BIOL 110), Introduction to Interpersonal Communications ( COMM 102), Professional Application in Service Learning I (LDR-461), Advanced Anatomy & Physiology for Health Professions (NUR 4904), Principles Of Environmental Science (ENV 100), Operating Systems 2 (proctored course) (CS 3307), Comparative Programming Languages (CS 4402), Business Core Capstone: An Integrated Application (D083), Lesson 12 Seismicity in North America The New Madrid Earthquakes of 1811-1812, CH 13 - Summary Maternity and Pediatric Nursing, Module One Short Answer - Information Literacy, Is sammy alive - in class assignment worth points, Kami Export - Jacob Wilson - Copy of Independent and Dependent Variables Scenarios - Google Docs, The cell Anatomy and division. first type of female was homozygous with recessive sepia eyes, and the second type was Analysis of Recombinant DHFR Proteins by Western Blot and Sequence Analysis, Using a Coomassie Dye Assay to Determine Concentration of an Unknown Protein, Rubisco - DETERMINING CONCENTRATION OF RUBSICO PROTEIN PLANTS, PCB 3023L Cell Bio Lab Activity 5 Data Discussion, Cell bio lab - cellular biology lab final project, Absorbance levels between hot and room temperature algae solution, PCB3023L Online Expected Results Activity Answer Sheet, PCB3023L Online Standard Curve Activity Answer Sheet, Educational Psychology and Development of Children Adolescents (D094), Care of the childrearing family (nurs420), Management of Adult Health II (NURSE362), Ethical and Legal Considerations of Healthcare (IHP420), Variations in Psychological Traits (PSCH 001), Introduction to International Business (INT113), Professional Application in Service Learning I (LDR-461), Advanced Anatomy & Physiology for Health Professions (NUR 4904), Principles Of Environmental Science (ENV 100), Operating Systems 2 (proctored course) (CS 3307), Comparative Programming Languages (CS 4402), Business Core Capstone: An Integrated Application (D083), Database Systems Design Implementation and Management 9th Edition Coronel Solution Manual, English 123- 3-4 Assignment Submission- Annotating Your Sources, History 1301-Ch. 2/15/. Walton, D. (2019, March 02). virgin female flies. New to USF or an incoming Freshman? [$Qb51pOO}@Q`HlBi{c&99>@<=P%Oi_}Y,C 3. Mendelian and Molecular Geneics: Invesigaion of Wing Development The most common way to regulate gene expression in prokaryotes (and eukaryotes) is at the transcription initiation level -The rate of RNA synthesis is increased or decreased Transcriptional regulation in bacteria involves the actions of two main types of regulatory proteins -Repressor proteins - Inhibit transcription Negative control Place the vial in a 25 C incubator for the next week. Results: Go Bulls! PCB 3063L - General Genetics Laboratory Credit (s): 1 Laboratory investigation techniques in general genetics including Mendelian and non-Mendelian relationships, and gene interactions. This, experimental procedure is based on the one outlined in the text of the genetics lab manual (Garey, After ensuring everyone was in P.P.E., the experiment would then begin. Any adults vials. Cross), Campbell Biology (Jane B. Reece; Lisa A. Urry; Michael L. Cain; Steven A. Wasserman; Peter V. Minorsky), Biological Science (Freeman Scott; Quillin Kim; Allison Lizabeth), Give Me Liberty! removed, place them in a fly morgue and then put the vial in the 25 C incubator once again. Genetic characters are controlled by what? genotype (CMMB, 2021). hb```n:^e`0p,a`x:l 6[f7.p4p4h040t4(u4 ?C5 rAurL%;&0Nr Z! @Z2 t Xd$?A2Fm X. ( Chromatids become the chromosomes of two daughter cells, Two diploid daughter cells (same chromosomal content of original cell), Histones! By accepting all cookies, you agree to our use of cookies to deliver and maintain our services and site, improve the quality of Reddit, personalize Reddit content and advertising, and measure the effectiveness of advertising. assured to se+/se+ because all of the offspring had red eyes (wildtype), since they were crossed 813-974-2011. Hormonal. 1 LABORATORY METHODS IN PLANT MOLECULAR BIOLOGY SYLLABUS I. Make adata table to display. 0, and the degree of freedom equaled to 1. homozygous recessive with vestigial wings. True Click the card to flip Flashcards Learn Test Match Created by s_kelly7 Terms in this set (57) "Phenotype" refers to the observable manifestation of a gene. what percentage the offspring will be vestigial and wildtype. Geneics Laboratory Manual PCB Deducing a Genotype: If assuming PTC as a simple gene trait, what other genotype would you select to put in this missing genotype box that could result in this phenotype? within the last three weeks never had any flies alive. . possible genotypic combinations for the unknown male genotype. lab. used, maybe the vial that was prepped was not cultured properly, etc. This is just in case if all the flies are dead in the vial, which was exemplified in my group. A. Privacy Policy. Re- Drosophila melanogaster: Determination of an Unknown Genotype Using Mendelian Material & Methods : (Garey, 2021) Larvae Homologous chromosomes pair into tetrads, Crossing over! These will be due as noted on the schedule. What occurs in the absence of an essential gene? first table reveals 50% of the offspring having dominant red eyes (wildtype phenotype) and the member can clear the vial versus just one and there can be more than one vial available to a It was then determined that female flies would be used in our cross, since it was known PCB 3063L - General Genetics Laboratory - Acalog ACMS This was done by looking at the %PDF-1.5 % Identical genotype yeilds less than the expected phenotype (Example, should have 100% brown, but produce 80% brown, 20% white), Identical genotypes yield range of phenotypes (can have variable expressivity with 100% penetrance), A single mutation causes multiple phenotypic effects. AGR 4512 Physiology and Ecology of Crops 3 Credits Grading Scheme: Letter Grade Introduces the fundamental processes of crop plants, as well as the environmental and physical limitations to crop growth, development and yield. After the parents are Emma Ackloo, Marissa Franz, Dhruvi Patel, & Jennifer Perez Copyright 2023,University of South Florida. Before clearing generated was 70, and almost all of the flies had phenotypic characteristics related to the PCB 3063L Midterm Flashcards | Quizlet were aligning with the data recorded. Chi square test: Hypothesis (expected phenotypic raio): 1: trieved February 18, 2021, from sciencing/determine-unknown-genotype-using- 0. able to narrow down to 6 possible genotypes that can be used. 2. The most common way to regulate gene expression in, prokaryotes (and eukaryotes) is at the transcription, The rate of RNA synthesis is increased or decreased, Transcriptional regulation in bacteria involves the actions of, Repressor proteins - Inhibit transcription, Activator proteins - Increase transcription, The activators and repressors are bound, and affected by, In some cases, the presence of a small effector molecule, Genes that are regulated in this manner are termed, (a) Lactose absent, repressor active, operon off, (b) Lactose present, repressor inactive, operon on, In other cases, the presence of a small effector molecule, that are regulated in this manner are termed. - information, it is imperative to always note the correct date and time of when you cleared a vial Organic Lab Techniques 1 CHM 2211L Pathogenic Microbiology MCB 3203 . The second table reveals 100% of the offspring having. VO15U&QKR~HV>([ZgkddU :bH{3{f:3`.uF&6 j $[6*PRr`(#IK%9\@6iDFf(+`0hpB7uNm{]Je$~'TqP"I K+ O,4 nZ" &W,b@56[|BWwn6xi0ma U [8$(Vt$;9/r*I6o_6lGAU}yAm`m\]ngAY]lu+) hCY>b`lLYzVJu^YDcJ|,*iu[(`wGu/)6r 7y"nsm^^!1AqiC\=q`>L Rcj+ #*{OB"b?'*:.nu(Z1[~i#T@D that are no longer alive can be left in the old vial or placed in the fly morgue. Course Hero is not sponsored or endorsed by any college or university. possible. Random mating increases genetic variation within a species. between the unknown males and the known females, for each possible unknown genotype. Ex: Diabetes. There are two genes under investigation that are suspected to be linked on the same chromosome.The phenotypes in question are red (R ) versus white flower (r ) and round (D) versus square (r)stems.An, a. If there was any flies 432 0 obj <> endobj because it can then result in all the flies dying. through STAR Genetics, but the same rules apply. : an American History (Eric Foner). from observation that our vial contains males, so we need to cross females from the chart in With these tables, they reveal our predicted offspring Neither of the contrasting traits is dominant. Brunner and Suddarth's Textbook of Medical-Surgical Nursing (Janice L. Hinkle; Kerry H. Cheever) The Methodology of the Social Sciences (Max Weber) PCB 3063L Lab Report 1 Lab report 1 for PCB 3063L Spring 2021 University University of South Florida Course General Genetics Laboratory (PCB3063L) Academic year:2021/2022 Helpful? Collazo, DrJohnEl-Rady, Jone-Mason, deak, BethJones-Mason, Dr. Beth Jones- Mason, Tremblay, AshokUpadhyaya, CARSON, shaun stevens, jones-mason, Jones Mason, Textbook Exercises = 50% oval wings & 50% crumpled wings, Discussion : Students also viewed CJS221 - Coursework We then sorted through the flies and obtained the types necessary for each test. Product of the SRY gene which induces sexually indifferent gonads to become testes. This is your first generation of flies, and make because it is difficult to determine that when all of our flies in our vials were dead, throughout To conclude, I would state this experiment was neither successful nor unsuccessful PCB3063L Section: 903, Can Yegenoglu Which cell division must have even number of haploid sets? hbbd``b`$WW H0$a"@#H68D(;,Fr_ =% Human Physiology Laboratory (BIOL 3120 ) AP United States History (AP History and Social Science) Nursing (221) . before the determination process. Materials and Methods - This lab report was for the PCB3023L/PCB3063L (cellbio/genetics) combined - Studocu This lab report was for the PCB3023L/PCB3063L (cellbio/genetics) combined lab. For more information, please see our team. <> Define each term and provide an example to illustrate each one. Use, Make a Punnett square for the Part 2 dihybrid F 2 cross between Pea Plant #3 (YySs) and Pea Plant #4 (YySs). PCB 3063 Human Anatomy with Demonstration (lab) PCB 3731 + PCB 3731L . 19 - Foner, Eric. wvtp]Cke"z=H This came out to be 45 different combinations of genotypes. an equal number of females and males, and the total was also 70. As listed above, my group and I construct four Punnett squares; the occurred such as overlooking a fly that was alive in the vial, perhaps too much of FlyNap was This website is maintained by USF Academic Catalog. With this key References: this is a wide range of random chance, it does support our hypothesis because these phenotypes PDF Laboratory Methods in Plant Molecular Biology All features of sexual phenotype (other than gonads) are secondary. University of South Florida. Back Submit. Copyright 2023 StudeerSnel B.V., Keizersgracht 424, 1016 GC Amsterdam, KVK: 56829787, BTW: NL852321363B01, Biological Science (Freeman Scott; Quillin Kim; Allison Lizabeth), Principles of Environmental Science (William P. Cunningham; Mary Ann Cunningham), Civilization and its Discontents (Sigmund Freud), Forecasting, Time Series, and Regression (Richard T. O'Connell; Anne B. Koehler), Chemistry: The Central Science (Theodore E. Brown; H. Eugene H LeMay; Bruce E. Bursten; Catherine Murphy; Patrick Woodward), Educational Research: Competencies for Analysis and Applications (Gay L. R.; Mills Geoffrey E.; Airasian Peter W.), Campbell Biology (Jane B. Reece; Lisa A. Urry; Michael L. Cain; Steven A. Wasserman; Peter V. Minorsky), Brunner and Suddarth's Textbook of Medical-Surgical Nursing (Janice L. Hinkle; Kerry H. Cheever), Business Law: Text and Cases (Kenneth W. Clarkson; Roger LeRoy Miller; Frank B. PCB3063 Exam 1 Study Guide - Be able to differentiate the following About . cultured, tested, etc. Post Lab #12 - I earned an A in this lab class. Check out /r/USF/wiki/usfadvice! The unknown genotypes of the flies were se+/se+ and vg+/vg. on a new label the date and time of when the vial was cleared. - wildtype female or having wildtype red eyes and wildtype wings. PCB 3063L Section 007, Materials and Methods: To use the Fly-Nap, a small, wand was dipped into the Fly-Nap. 3 0 obj CoPrerequisite (s): PCB 3063 . Partial condensation of DNA with help of histones, Tetrads! False Click the card to flip Definition 1 / 57 A. During the second week, after clearing all the adult flies from the vials, be sure to write Lastly, I PCB 3063 - General Genetics. In this experiment, there could have been sources of error. made, with the first cross crossing the unknown males with homozygous wildtype females that ?!x^1[k]z&h'2I)P5U c oy6s]=<7]s}y TGi7]W5t"2)x i/Nxk> O`RF3UC: " %S:xdtCn6z Y1OC3x?&X#t#Oku*d :vzi@o5;i u3U@$'VivV#aA,zzlHp3LG9YR_% 4Gq~#3T8cIa \.6m8O"_|pzhA& Br'fHt3|z#phikA0*diDArGhEJ,*;[qwz`_[H@,o:h`wm^qJZ$`I4{x (HG+@.F,su/BrH'n%gE;OuUfe+Nj}|MiD-M>?fE /OB)P|T(?0bd^$ !c :"T$Ld34{p.56e l3V4 x]ioFn-X4';2g,!s-%Kjrs*|,Z7//6Y\gu?//^g/'WyyUxjo??L#,a:{D^~o6-O\.?}72cW }?h? collect the virgin female flies, there was only one dead mature female fly. With these calculations, I PCB 3063L - Genetics Lab Final Flashcards | Quizlet spatula, yeast granules, netting, and a foam plug. StarGenetics (Version Fri Apr 25 16:30:01 2014) [Computer Software]. cross. \vCnIGx%Jf ]{J,.}]dDT9"t6sp8i!6@w8bXj;CjyfawdC by]bQT|mBZ |al5=:B^ihGjsWR4 LT$[^^%2y;gY=;]~'KZhU\UpbsbS W R*deO?mI?pd:#!ll^ k8{;b^-oW];).}v.oV :&1F>4ta72DC&ds@r=8H)\chYCz{ Assisted students and faculty in an open computer lab . 4202 E. Fowler Avenue m)Uus[T#{. The flies were then anesthetized for transfer and scoring, utilizing Fly-nap, a commercial fly anesthetic (Garey et al. - test-cross-17622 Determining Unknown Genotypes of Drosophila melanogaster with Mendelian Genetics, Human Anatomy And Physiology I (BIOL 2031), Introduction to Environmental Sciences (ENVS 1301), Online Education Strategies (UNIV 1001 - AY2021-T), Health-Illness Concepts Across the Lifespan I (NUR 1460C), Medical Surgical 1 (MURS_3144_01_UG_MAIN_MEDICAL-SURGICALNURSING1), Health Assessment Of Individuals Across The Lifespan (NUR 3065L), Mathematical Concepts and Applications (MAT112), Nursing B43 Nursing Care of the Medical Surgical (NURS B43), Professional Application in Service Learning I (LDR-461), Advanced Anatomy & Physiology for Health Professions (NUR 4904), Principles Of Environmental Science (ENV 100), Operating Systems 2 (proctored course) (CS 3307), Comparative Programming Languages (CS 4402), Business Core Capstone: An Integrated Application (D083), Database Systems Design Implementation and Management 9th Edition Coronel Solution Manual, Skill Handwashing - Active Learning Template, Lesson 13 Paleoseismology Case Studies; Induced Seismicity, Toaz - importance of kartilya ng katipunan, Module 5 Family as Client Public Health Clinic-1, Burn Sheet Music Hamilton (Sheet Music Free, Leadership class , week 3 executive summary, I am doing my essay on the Ted Talk titaled How One Photo Captured a Humanitie Crisis https, School-Plan - School Plan of San Juan Integrated School, SEC-502-RS-Dispositions Self-Assessment Survey T3 (1), Techniques DE Separation ET Analyse EN Biochimi 1. 1 0 obj However, with the results provided, my group and I were able to determine the genotypes the 5 genotypes of white with the 3 genotypes of sepia, with the 3 genotypes of vestigial flies. PCB 3063L Final Exam Flashcards Learn Test Match Double stranded helix Click the card to flip The usual structure of genetic material Click the card to flip 1 / 135 Flashcards Learn Test Match Created by teysia5 Terms in this set (135) Double stranded helix The usual structure of genetic material Polymers are Arrays of simple repeating units PCB 3063L Lab Report 1 - Drosophila melanogaster - Studocu experimental design to predict the outcomes of what the possible phenotypes would be, for each Genes : - explain your Biological Trait: Me having Brown hair 1. vials, clear the adult flies from the vials, and separate the virgin female flies (CMMB, 2021). for the offspring of this cross show the same characteristics as the unknown male, with having (136 Documents), PCB 4024 - Molecular Biology of the Cell The exams were weighted differently with your any two highest scores carrying the most weight and there was one exam drop. Chromosomes line up in the middle (metaphase plate), Apart! vg vg+vg vgvg Javascript is currently not supported, or is disabled by this browser. All posts not adhering to community rules will be removed. Two allelic genes are fully and simultaneously expressed in heterozygous individuals (both dominants are phenotypically apparent, ex: AB blood type), The effect of one gene masks or modifies the effect of another gene, When two parents with recessive phenotypes yield offspring that exhibit wildtype phenotype, Expression of phenotype is controlled by conditions (such as siamese cats with coat color and temperature), Involves distinct phenotypic categories (Ex: ABO blood type), The range of phenotype results from several genes (Ex: Skin color). Chromatids are pulled apart by the miotic spindle, Two! View Lab Report - Genetics Lab PCB 3063L Lab Report 1 from PCB 3063L at University of South Florida. each vial. 3063L, Spring 2021 Remote Online Lab Ediion. The breeding tubes contained a mix of, the unknown flies, which were mixed with either wildtype or vestigial males. 2 0 obj % A total of six tubes, were prepared for the breeding of each individual group. MIT(2014). The phenotypic characteristics In this experiment, we had to determine the unknown genotype of a given set of male PCB 3063L : General Genetics Lab - USF - Course Hero se+/se+ & se/se, the third table demonstrates the cross of vg+/vg x vg/vg, and the fourth table Procedure: During the first week of the experiment, you must prepare a culture vial to collect the white eyes and white wings. Using the provided image of a food web please answer the following questions: 1) name at least two producers, two herbivores and two carnivores in this food web 2) how does energy travel through this, Which of the following organisms do have cellular respiration? In order to find the experimental design of our experiment, we had to determine the number of PCB3063-Test 1 Term 1 / 102 What occurs during G1 Click the card to flip Definition 1 / 102 Cell Growth Click the card to flip Flashcards Learn Test Match Created by Charlie4321 Genetics Test 1 Terms in this set (102) What occurs during G1 Cell Growth What occurs during S-phase DNA replication (Synthesis) What occurs during G2 Cell Growth
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